博来霉素所致小鼠硬皮病模型中通过AQP3介导的H2O2转运入成纤维细胞使得TGF-β活化

Activation of TGF-β1 by AQP3-Mediated H2O2 Transport into Fibroblasts of a Bleomycin-Induced Mouse Model of Scleroderma
2016-12-21 19:00发表评论
作者:Luo, J., Liu, X., Liu, J., Jiang, M., Luo, M., Zhao, J.
机构: 同济大学医学院,同济医院皮肤科
期刊: J Invest Dermatol2016年12月12期136卷

Hydrogen peroxide (H2O2), the most important reactive oxygen species, mediates intracellular signal transmission and is transported into cells by aquaporin 3 (AQP3). However, it remains unclear whether AQP3 is involved in the pathogenesis of scleroderma. In this study, we examined the role of AQP3 in a bleomycin-induced mouse model of scleroderma. We observed that H2O2 and AQP3 levels in mouse skin increased with the bleomycin injection period relative to phosphate-buffered saline-injected control mice. AQP3 mRNA and protein levels were higher in bleomycin mice fibroblasts than in phosphate-buffered saline mice fibroblasts, and AQP3 immunofluorescence signals in the cytoplasm and cell membrane of bleomycin mice fibroblasts were much stronger than those in phosphate-buffered saline mice fibroblasts. Bleomycin-induced increases in H2O2, transforming growth factor-β1, collagen type I, and collagen type III levels in bleomycin mice fibroblasts were blocked by silencing AQP3. In addition, silencing AQP3 decreased H2O2 levels, transforming growth factor-β1 expression, and fibrosis in the scleroderma mouse model. These results demonstrate that AQP3-mediated transport of H2O2 into bleomycin mice fibroblasts activated transforming growth factor-β1, and silencing AQP3 is a potential approach for treating scleroderma. © 2016 The Authors

通讯机构:Department of Dermatology, Tongji Hospital, Tongji University School of Medicine, Shanghai, China
学科代码:基础医学 皮肤病学   关键词:博来霉素 小鼠硬皮病 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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