冬虫夏草水溶剂对氧嗪酸钾与次黄嘌呤所致高尿酸血症小鼠的作用

Actions of water extract from Cordyceps militaris in hyperuricemic mice induced by potassium oxonate combined with hypoxanthine
2016-12-28 16:18发表评论
作者:Yong, T. , Zhang, M., Chen, D., Shuai, O., Chen, S., Su, J., Jiao, C., Feng, D., Xie, Y.
机构: 华南应用微生物国家重点实验室,广东省菌种保藏与应用重点实验室暨广东省应用微生物开放实验室,广东省微生物研究所
期刊: J Ethnopharmacol2016年12月24期194卷

Ethnopharmacological relevance Cordyceps militaris was recorded in the classic traditional Chinese medicine book with the main functions of “protecting liver and enhancing kidney functions”, influencing serum uric acid levels. Aim of study The aim is to investigate the hypouricemic effects and possible mechanism of C. militaris in hyperuricemic mice. Materials and methods A water extract (WECM) was prepared by decocting C. militaris directly at 80 °C in water bath, followed by lyophilization. WECM at 50, 100 and 200 mg/kg was orally administered to hyperuricemic mice induced by potassium oxonate and hypoxanthine combinedly and allopurinol (5 mg/kg) was served as a positive control. Results WECM exhibited excellent hypouricemic activity, which could decrease the serum uric acid levels of the hyperuricemic mice (306 μmol/L) to 189, 184 and 162 μmol/L at different doses respectively (P<0.01), approaching the levels of normal mice (184 μmol/L). The urate transporter 1 (URAT1) protein levels of kidney at different doses of WECM were 28.15, 17.43, 9.03 pg/mL respectively, much lower than that in the hyperuricemia group (93.45 pg/mL, P<0.01); and suggested WECM may interact with URAT1. Docking simulations using modeled structure of URAT1 suggested that LYS145, ARG325, ARG477 and ASP168 of URAT1 are key functional residues of URAT1. Four active compounds in C. militaris were identified and their interaction energies with target were estimated between −200 and −400 kcal/mol. Conclusions These findings suggested that C. militaris produced significant hypouricemic actions and the hypouricemic effects of WECM may be attributed to the inhibitive effect of WECM on URAT1 protein levels. The results of blood urine nitrogen and serum creatinine levels and liver, kidney and spleen coefficients showed that WECM have no negative impacts on liver, renal and spleen functions. The screened four active compounds using molecular docking method deserve further investigation in other work. © 2016 Elsevier Ireland Ltd

通讯机构:State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application and Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China
学科代码:内科学 补充和替代医学 药学   关键词:冬虫夏草水溶剂 氧嗪酸钾 次黄嘌呤 高尿酸血症 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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