苦地丁在体内及体外均可通过抑制NF-κB信号通路而减弱LPS所致的炎症反应

Corydalis bungeana Turcz. attenuates LPS-induced inflammatory responses via the suppression of NF-κB signaling pathway in vitro and in vivo
2017-01-05 14:20发表评论
作者:Zhai, X.-T., Chen, J.-Q., Jiang, C.-H., Song, J., Li, D.-Y., Zhang, H., Jia, X.-B., Tan, W., Wang, S.-X., Yang, Y., Zhu, F.-X.
机构: 南京中医药大学中西医结合学院
期刊: J Ethnopharmacol2016年12月24期94卷

Ethnopharmacological relevance Corydalis bungeana Turcz. (C. bungeana) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host. Aim of the study we studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment. Materials and methods Anti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-qPCR, respectively. Phosphorylation status of IκBα and p65 was illustrated by western blot assay. Results C. bungeana reduced the secretion of NO, TNF-α, IL-6 and IL-1β through inhibiting the protein expression of iNOS, TNF-α, IL-6 and IL-1β in vitro and in vivo. Western blot analysis suggested that C. bungeana supressed NF-κB activation via regulating the phosphorylation of IκBα and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue. Conclusions The results indicate that C. bungeana supresses the activation of NF-κB signaling pathway through inhibiting phosphorylation of IκBα and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro. © 2016 Elsevier Ireland Ltd

通讯机构:Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China
学科代码:内科学 补充和替代医学 药学   关键词:苦地丁 NF-κB信号通路 减弱LPS 炎症反应 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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