非小细胞肺癌患者支气管刷检发现MCM7表达的预后意义
State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China
Purpose: To identify potential biomarkers for the prognosis of non-small cell lung cancer (NSCLC) patients by using bronchial brushing specimens. Methods: The expression of MCM7, Ki67 and EGFR was evaluated in 494 NSCLC tissues and 174 bronchial brushings using immunohistochemical and immunocytochemical techniques. Associations between protein expression and clinico-pathologic parameters were assessed, and the impact on overall survival (OS) was analyzed. Results: High expression of MCM7, Ki67 and EGFR was detected in 33.3%, 23.5% and 12.7% of tissues and in 52.4%, 52.7% and 20.6% of bronchial brushings, respectively. Expression of MCM7 and Ki67 was associated with squamous cell carcinoma (SCC) in both tissues and bronchial brushings (MCM7: P= 0.0007, 0.00003; Ki67: P< 0.00001, 0.00001). Overexpression of MCM7 in tumor tissues was detected more frequently in poorly differentiated tumors (P= 0.0120) and non-bronchioloalveolar carcinomas (non-BACs) (P= 0.0238). EGFR overexpression was observed in tissues of larger tumors (P= 0.00004) and in bronchial brushings at later stage (P= 0.0262). Kaplan-Meier curves indicated that patients with overexpression of MCM7 or Ki67 had a poorer OS compared to those with low expression for all stages (P< 0.00001, 0.0233) and early-stages (P< 0.00001, 0.0032). In particular, the patients with MCM7 overexpression in bronchial brushings had a poorer prognosis (P= 0.0045). Multivariate Cox regression analysis showed that MCM7 was an independent prognostic indicator both in tissue samples and bronchial brushings. Conclusions: Our data suggest that MCM7 and Ki67 in tumor tissues may be potential markers of a poor prognosis for NSCLC patients. MCM7 in bronchial brushings also showed an independent prognostic value, which may be useful when biopsies are unavailable. © 2012 Elsevier Ireland Ltd.
Wang, M.-R.; State Key Laboratory of Molecular Oncology, Cancer Institute/Hospital, PUMC/CAMS, 17 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China; email: wangmr07@gmail.com
来源: Scopus
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