|
|
|
COX催化的花生四烯酸过氧化反应产生的新自由基的特征 |
|
|
|
|
|
Characterization of novel radicals from COX-catalyzed arachidonic acid peroxidation |
|
|
|
|
|
Yu Q, Purwaha P, Ni K, Sun C, Mallik S, Qian SY 2009/8/4 15:32:00 |
|
【发表评论】
|
|
|
|
打印|
推荐给好友
|
|
|
Free Radical Biology and Medicine, 2009, Volume 47, Issue 5
|
|
|
|
|
|
|
The peroxidation of arachidonic acid (AA) catalyzed by cyclooxygenase (COX) is a well-known free radical-mediated process that forms many bioactive products. Because of a lack of appropriate methodologies, however, no comprehensive structural evidence has been found previously for the formation of COX-mediated and AA-derived free radicals. Here we have used a combination of LC/ESR and LC/MS with a spin trap, α-[4-pyridyl-1-oxide]-N-tert-butylnitrone (POBN), to characterize the carbon-centered radicals formed from COX-catalyzed AA peroxidation in vitro, including cellular peroxidation in human prostate cancer cells (PC-3). Three types of radicals with numerous isomers were trapped by POBN as ESR-active peaks and MS-active ions of m/z 296, 448, and 548, all stemming from PGF2-type alkoxyl radicals. One of these was a novel radical centered on the carbon-carbon double bond nearest the PGF ring, caused by an unusual β-scission of PGF2-type alkoxyl radicals. The complementary nonradical product was 1-hexanol, another novel β-scission product, instead of the more common aldehyde. The characterization of these novel products formed from in vitro peroxidation provides a new mechanistic insight into COX-catalyzed AA peroxidation in cancer biology.
|
|
|
|
|
|
|
Correspondence Address: Qian, S.Y.; Department of Pharmaceutical Sciences, College of Pharmacy, Nursing, and Allied Sciences, North Dakota State University, Fargo, ND 58105, United States; email:steven.qian@ndsu.edu |
|
|
|
|
|
|
|
|
|
|
请登录后发表评论,点击此处登录。
|
|
|
疾病资源中心
王燕燕 王曙
上海交通大学附属瑞金医院内分泌科
患者,女,69岁。2009年1月无明显诱因下出现乏力,当时程度较轻,未予以重视。2009年3月患者乏力症状加重,尿色逐渐加深,大便习惯改变,颜色变淡。4月18日入我院感染科治疗,诉轻度头晕、心慌,体重减轻10kg。无肝区疼痛,无发热,无腹痛、腹泻、腹胀、里急后重,无恶性、呕吐等。入院半月前于外院就诊,查肝功能:ALT 601IU/L,AST 785IU/L,TBIL 97.7umol/L,白蛋白 41g/L,甲状腺功能:游离T3 30.6pmol/L,游离T4 51.9pmol/L,心电图示快速房颤。
医学数据库
|