中国作者重要发表 - 胎儿肺组织中1型11-β羟类固醇脱氢酶表达及白介素-1和皮质醇对其表达的调节



胎儿肺组织中1型11-β羟类固醇脱氢酶表达及白介素-1和皮质醇对其表达的调节





Expression of 11β-hydroxysteroid dehydrogenase type 1 in human fetal lung and regulation of its expression by interleukin-1β and cortisol





Yang Z., Zhu P., Guo C., Zhu X., Sun K. 2009/5/29 18:38:57

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Journal of Clinical Endocrinology and Metabolism, 2009, Volume 94, Issue 1




Context: Glucocorticoids are crucial in fetal lung function. The amount of cortisol available to its receptors is increased by 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1). Glucocorticoids and IL-1β are known to induce 11β-HSD1 expression in a number of tissues, but controversial results were obtained with regard to 11β-HSD1 expression in human fetal lung. Objective: We examined the expression of 11β-HSD1 and its regulation by cortisol and IL-1β in human fetal lung. Results: Immunohistochemistry revealed 11β-HSD1 expression in the epithelium and mesenchymal layer of the small bronchus and bronchiole of human fetal lung at 8 months but not at 4 months gestation, which was confirmed by PCR revealing 11β-HSD1 mRNA expression in the fetal lung tissue. By using a cell line derived from human fetal lung fibroblasts, we demonstrated that cortisol (10-5 to 10-3 mmol/liter) or IL-1β (0.1 to 10 ng/ml) induced 11β-HSD1 mRNA expression in a concentration-dependent manner. The induction of 11β-HSD1 by IL-1β was further increased by cortisol, whereas the induction of cyclooxygenase 2 by IL-1β was inhibited by cortisol. Nuclear factor κB activation inhibitor could only block the induction of cyclooxygenase 2 but not 11β-HSD1 by IL-1β, suggesting that different mechanisms were utilized by IL-1β in the regulation of 11β-HSD1 versus proinflammatory mediators. Global inhibition of CCAAT-enhancer-binding proteins (C/EBPs) with transfection of C/EBP-specific dominant-negative expression plasmid could attenuate the induction of 11β-HSD1 by IL-1β, suggesting that C/EBPs may mediate the induction of 11β-HSD1 by IL-1β. Conclusions: 11β-HSD1 is expressed in human fetal lung; cortisol and IL-1β could synergistically induce its expression. Copyright © 2009 by The Endocrine Society.



Correspondence Address: Sun, K.; School of Life Sciences, Fudan University, 220 Handan Road, Shanghai 200433, China; email: sungang@fudan.edu.cn



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高血压	慢性阻塞性肺病
脂肪性肝病	糖尿病
卒中	肺癌
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甲状腺功能亢进合并黄疸

王燕燕王曙

上海交通大学附属瑞金医院内分泌科

患者，女，69岁。2009年1月无明显诱因下出现乏力，当时程度较轻，未予以重视。2009年3月患者乏力症状加重，尿色逐渐加深，大便习惯改变，颜色变淡。4月18日入我院感染科治疗，诉轻度头晕、心慌，体重减轻10kg。无肝区疼痛，无发热，无腹痛、腹泻、腹胀、里急后重，无恶性、呕吐等。入院半月前于外院就诊，查肝功能：ALT 601IU/L，AST 785IU/L，TBIL 97.7umol/L，白蛋白 41g/L，甲状腺功能：游离T3 30.6pmol/L，游离T4 51.9pmol/L，心电图示快速房颤。

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