中国作者重要发表 - 冷冻保存人类卵巢组织时采用针浸润玻璃化可使所需的冷冻保护剂浓度降低



冷冻保存人类卵巢组织时采用针浸润玻璃化可使所需的冷冻保护剂浓度降低





Needle immersed vitrification can lower the concentration of cryoprotectant in human ovarian tissue cryopreservation





Xiao Z, Wang Y, Li L, Luo S, Li S-W 2010/11/22 13:30:00

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Fertility and Sterility, 2010, Volume 94, Issue 6




Objective: To investigate whether needle immersed vitrification (NIV) can further lower the concentration of cryoprotective agent (CPA). Design: Experimental cross-sectional controlled in vitro study. Setting: University teaching hospital. Patient(s): Human ovarian biopsy tissues were obtained from ten women undergoing gynecology operations. Intervention(s): Ovarian cortical tissues were cryopreserved using slow freezing or vitrification. The vitrification solutions used were as follows: group A: 2.69 mol/L ethylene glycol (EG) + 2.11 mol/L dimethylsulfoxide (DMSO) + 0.5 mol/L sucrose; group B: 2.42 mol/L EG + 1.90 mol/L DMSO + 0.5 mol/L sucrose; group C: 2.15 mol/L EG + 1.69 mol/L DMSO + 0.5 mol/L sucrose; and group D: 1.88 mol/L EG + 1.48 mol/L DMSO + 0.5 mol/L sucrose. Main Outcome Measure(s): Histologic evaluations were performed using light and electron microscopy. Apoptosis was assessed by TUNEL staining. Tissue damage after cryopreservation was measured by the levels of lactate dehydrogenase (LDH) in culture. Result(s): The proportion of normal ultrastructure of granulosa cells and stromal cells in groups B and C was higher than that in group A. The proportion of TUNEL-positive primordial follicles and stromal cells in the NIV groups decreased with reduction of concentration. Additionally, LDH levels in groups B and C were lower than in group A. Conclusion(s): The NIV method could further lower the concentration of CPA. Therefore, we can use the CPA of group C as an optimal concentration for NIV. Copyright © 2010 American Society for Reproductive Medicine, Published by Elsevier Inc.



Correspondence Address: Li, S.-W.; Reproductive Medical Center, West China Second University Hospital, Sichuan University, Post Code 610041, Ren Min Nan Lu, City of Chengdu, Sichuan, China; email:lishangwei17@gmail.com



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