中国作者重要发表 - SOCS-3基因过度表达通过JEG-3滋养细胞促进 IL-10 的产生



SOCS-3基因过度表达通过JEG-3滋养细胞促进 IL-10 的产生





Over-expression of SOCS-3 Gene Promotes IL-10 Production by JEG-3 Trophoblast Cells





Dong Q., Fan R., Zhao S., Wang Y. 2009/5/29 18:39:42

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Placenta, 2009, Volume 30, Issue 1




Suppressor of cytokine signaling-3 (SOCS-3) plays an important role in negative regulation of inflammatory response. Evidence has shown that SOCS-3 and IL-10 expressions were significantly reduced in placental trophoblasts from preeclampsia. IL-10 is an anti-inflammatory cytokine. In this study, we sought to determine if enhance SOCS-3 expression could affect IL-10 production in placental trophoblasts. Placental JEG-3 cells were used. Over-expression of SOCS-3 was generated by transfection of JEG-3 cells with a green fluorescent protein (GFP) tagged SOCS-3 gene, SOCS-3/ZsGreen1, by siPORT lipid transfection. Cells transfected with ZsGreen1 vector only was used as control. Our results showed that IL-6 production was reduced in cells over-expressed with SOCS-3. Moreover, SOCS-3 transfected cells produced more IL-10 when stimulated with IL-6. The increased IL-10 production by JEG-3 cells was in a dose-dependent manner, p < 0.05. Our data suggested that enhanced SOCS-3 gene expression could promote IL-10 production by placental trophoblast cells, suggesting that SOCS-3 may play an important role in regulation of cytokine induced anti-inflammatory response in placental trophoblasts. © 2008 Elsevier Ltd. All rights reserved.



Correspondence Address: Wang, Y.; Department of Obstetrics and Gynecology, Louisiana State University Health Sciences Center, Shreveport, LA 71130, United States; email: ywang1@lsuhsc.edu



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