E2-BSA通过PI 3K/ERK1/2和溶酶体降解通路激活caveolin-1并促进EPC增殖
E 2 - BSA activates caveolin-1 via PI 3K/ERK1/2 and lysosomal degradation pathway and contributes to EPC proliferation
Department of Physiology, Zhongshan School of Medicine-Sun Yat-sen University, 74 Zhongshan Road 2, Guangzhou, 510080, China
Background: The mechanism that estrogen (E 2) increases the number of endothelial progenitor cells (EPC) is largely unknown. Here we used E 2-conjugated bovine serum albumin (E 2-BSA, membrane impermeable) to investigate whether the membrane estrogen receptor (mER) and its related protein caveolin-1 (CAV-1) are involved in these processes. Methods and results: E 2-BSA promoted [ 3H]-thymidine incorporation of EPC through increasing CAV-1 expression via mER (ERα, but not ERβ or GPR30). Both cholesterol depletion and CAV-1 knockdown with use of CAV-1 siRNA significantly attenuated E 2-BSA-induced [ 3H]-thymidine incorporation. Western blot showed that E 2-BSA increased membrane CAV-1 protein expression 12 h after treatment, whereas mRNA levels of CAV-1 were augmented until 24 h after E 2-BSA treatment. Furthermore, pre-incubated EPC with ICI 182780 (a specific ER antagonist), LY 294002 (a selective PI 3K inhibitor) or PD 98059 (a specific ERK1/2 inhibitor) before E 2-BSA inhibited the late-stage effect of E 2-BSA (≥ 24 h) on up-regulation of CAV-1 mRNA and protein expression. Pulse chase results demonstrated that E 2-BSA inhibited lysosome-mediated degradation of CAV-1 protein at the early stage (≤ 12 h), and then resulted in the increased CAV-1 protein. Conclusion: In the present work we demonstrated that E 2-BSA promotes EPC proliferation through mER-ERα in CAV-1-dependent manner: prolonging the stability of CAV-1 protein through quick inhibition of the lysosomal degradation pathway at the early stage (≤ 12 h) and up-regulating CAV-1 at transcription levels through PI 3K/ERK1/2 signaling pathway at the late stage (≥ 24 h). These data indicated that a there is a novel mechanism of E 2-BSA in the regulation of EPC proliferation through CAV-1.
学科代码:心血管病学 关键词:E2-BSA通过PI 3K/ERK1/2和溶酶体降解通路激活
来源: Scopus
来源: Scopus
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