谷氨酸促进幼猪空肠的紧密连接蛋白表达并调节促肾上腺皮质激素释放因子信号通路

Glutamine enhances tight junction protein expression and modulates corticotropin-releasing factor signaling in the jejunum of piglets
2015-06-19 09:00发表评论
作者:Wang, H., Zhang, C., Wu, G., Sun, Y., Wang, B., He, B., Dai, Z., Wu, Z.
机构: 中国农业大学动物营养学国家重点实验室
期刊: J NUTR2015年4月1期145卷

Background: Dysfunction of tight junction integrity is associated with decreased nutrient absorption and numerous gastrointestinal diseases in humans and piglets. Although L-glutamine has been reported to enhance intestinal-mucosal mass and barrier function under stressful conditions, in vivo data to support a functional role for L-glutamine on intestinal tight junction protein (TJP) expression in weanling mammals are limited. Objective: This study tested the hypothesis that glutamine regulates expression of TJPs and stress-related corticotropinreleasing factor (CRF) signaling in the jejunum of weanling piglets. Methods: Piglets were reared by sows or weaned at 21 d of age to a corn and soybean meal-based diet that was or was not supplemented with 1% L-glutamine for 7 d. Growth performance, intestinal permeability, TJP abundance, and CRF expression were examined. Results: Weaning caused increases (P < 0.05) in intestinal permeability by 40% and in CRF concentrations by 4.7 times in association with villus atrophy (P < 0.05). Western blot analysis showed reductions (P < 0.05) in jejunal expression of occludin, claudin-1, zonula occludens (ZO) 2, and ZO-3, but no changes in the abundance of claudin-3, claudin-4, or ZO-1 in weanling piglets compared with age-matched suckling controls. Glutamine supplementation improved (P < 0.05) intestinal permeability and villus height, while reducing (P < 0.05) jejunal mRNA and protein levels for CRF and attenuating (P < 0.05) weanling-induced decreases in occludin, claudin-1, ZO-2, and ZO-3 protein abundances. Conclusion: Collectively, our results support an important role for L-glutamine in regulating expression of TJPs and CRF in the jejunum of weanling piglets. © 2015 American Society for Nutrition.

 

 

通讯机构: State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, China
学科代码:内科学 儿科学   关键词:谷氨酸 幼猪空肠 蛋白 调节促肾 腺皮质激素 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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