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| 3',5'-cAMP反应元件结合蛋白上调与小鼠卵泡刺激素诱导的卵细胞成熟相关的细胞色素P450甾醇14-α-去甲基酶表达 |
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| 3′,5′-cyclic adenosine monophosphate response element binding protein up-regulated cytochrome P450 lanosterol 14α-demethylase expression involved in follicle-stimulating hormone-induced mouse oocyte maturation |
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| Ning G., Ouyang H., Wang S., Chen X., Xu B., Yang J., Zhang H., Zhang M., Xia G. 2009/5/29 18:38:57 |
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Molecular Endocrinology, 2008, Volume 22, Issue 7
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| Cytochrome P450 lanosterol 14α-demethylase (CYP51) is a key enzyme in sterols and steroids biosynthesis that can induce meiotic resumption in mouse oocytes. The present study investigated the expression mechanism and function of CYP51 during FSH-induced mouse cumulus oocyte complexes (COCs) meiotic resumption. FSH increased cAMP-dependent protein kinase (PKA) RIIβ level and induced cAMP response element-binding protein (CREB) phosphorylation and CYP51 expression in cumulus cells before oocyte meiotic resumption. Moreover, CYP51 and epidermal growth factor (EGF)-like factor [amphiregulin (AR)] expression were blocked by 2-naphthol-AS-Ephosphate (KG-501) (a drug interrupting the formation of CREB functional complex). KG-501 and RS21607 (a specific inhibitor of CYP51 activity) inhibited oocyte meiotic resumption, which can be partially rescued by progesterone. These two inhibitors also inhibited FSH-induced MAPK phosphorylation. EGF could rescue the suppression by KG-501 but not RS21607. Furthermore, type II PKA analog pairs, N6-monobutyryl- cAMP plus 8-bromo-cAMP, increased PKA RIIβ level and mimicked the action of FSH, including CREB phosphorylation, AR and CYP51 expression, MAPK activation, and oocyte maturation. All these data suggest that CYP51 plays a critical role in FSH-induced meiotic resumption of mouse oocytes. CYP51 and AR gene expression in cumulus cells are triggered by FSH via a type II PKA/CREB-dependent signal pathway. Our study also implicates that CYP51 activity in cumulus cells participates in EGF receptor signaling-regulated oocyte meiotic resumption. Copyright © 2008 by The Endocrine Society. |
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| Correspondence Address: Zhang, M.; College of Biological Sciences, China Agricultural University, Beijing 100094, China |
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 疾病资源中心
摘自：《西氏内科学》，第23版
患者女性，21岁，因干咳、间歇性气促2个月到急诊科就诊。开始症状为上呼吸道感染引起的鼻塞、流涕和咳嗽。医生检查后开了抗生素。服药后鼻部症状缓解，但仍有轻微干咳和呼吸困难。其他症状包括疲劳和焦虑。否认发热、体重减轻、胸痛、端坐呼吸、气喘、鼻后滴漏、胃灼热以及神经系统症状。
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