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| 小鼠睾丸支持细胞TOLL样受体的表达模式和功能 |
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| Expression patterns and functions of toll-like receptors in mouse sertoli cells |
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| Wu H., Wang H., Xiong W., Chen S., Tang H., Han D. 2009/5/29 18:38:57 |
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Endocrinology, 2008, Volume 149, Issue 9
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| Toll-like receptors (TLRs) play crucial roles in mediating innate and adaptive immunity. Sertoli cells create a microenvironment that protects seminiferous tubules from autoantigens and invading pathogens. Here we examined the expression and potential function of TLR family in mouse Sertoli cells. RT-PCR, Western blotting, and flow cytometry were used to analyze gene expression. Immunofluorescence staining was used to determine activation of nuclear factor-κB. ELISA was used to detect secreted cytokines in culture medium. The phagocytosis assay was performed by Oil Red O staining for lipid droplets. We demonstrated that TLR2, TLR3, TLR4, and TLR5 are highly expressed; TLR6, TLR7, and TLR13 are expressed at relatively low level; and TLR1, TLR8, TLR9, TLR11, and TLR12 are not detected in mouse Sertoli cells. We focused our study on the roles of TLR2-TLR5 in Sertoli cells. Our data indicated that TLR2-TLR5 can be activated by their ligands in mouse Sertoli cells and subsequently increase expression of the inflammatory cytokines IL-1α, IL-6, and interferon-α, and -β. The augmented expression of the cytokines might be induced by activation of nuclear factor-κB. Notably, activation of TLR3 by its ligand, poly (I:C), specifically promoted phagocytosis of apoptotic spermatogenic cells by Sertoli cells. The TLR-induced Sertoli cell phagocytosis was found to be associated with the up-regulation of scavenger receptors. The results suggest that TLRs expressed in mouse Sertoli cells may play roles in defense against invasion of allo- and autoantigens in the seminiferous tubules. Copyright © 2008 by The Endocrine Society. |
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| Correspondence Address: Han, D.; Department of Cell Biology, Peking Union Medical College, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China; email: dshan@ibms.pumc.edu.cn |
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 疾病资源中心
摘自：《西氏内科学》，第23版
患者女性，21岁，因干咳、间歇性气促2个月到急诊科就诊。开始症状为上呼吸道感染引起的鼻塞、流涕和咳嗽。医生检查后开了抗生素。服药后鼻部症状缓解，但仍有轻微干咳和呼吸困难。其他症状包括疲劳和焦虑。否认发热、体重减轻、胸痛、端坐呼吸、气喘、鼻后滴漏、胃灼热以及神经系统症状。
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