自发性骨关节炎豚鼠的软骨下骨时空改变进展为显微镜下关节软骨退变

Spatial and temporal changes of subchondral bone proceed to microscopic articular cartilage degeneration in guinea pigs with spontaneous osteoarthritis
作者:Wang, T. , Wen, C.-Y. , Yan, C.-H. , Lu, W.-W. , C
机构: 香港大学李嘉诚医学院骨伤科
期刊: OSTEOARTHR CARTILAGE2013年4月4期21卷

Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong

 

Objective: This study aimed to investigate the spatial and temporal subchondral bone changeof Dunkin-Hartley (DH) strain guinea pigs spontaneous osteoarthritis (OA) model at early stage with three-dimensional Microfocal Computed Tomography (Micro-CT) analysis, histology and immunohistochemistry. Materials and methods: Knee joints of DH and Bristol Strain 2 (BS2)guinea pigs were analyzed at 1, 2 and 3 months of age for early staged subchondral boneultrastructure change of OA by Micro-CT and histology. And cartilage degeneration was monitored by histological examination. In addition, expression of Osterix was quantified by immunohistochemistry. Results: Microscopic cartilage degeneration was not found at first 3 months in both DH and BS2 guinea pigsSubchondral bone sclerosis with trabecular ultrastructure turnover was characterized in subchondral bone of DH guinea pigs. Increased thickness, bone mineral density with decreased porosity were defined in subchondral plate of DHguinea pigsSubchondral trabecular bone was found to be plate-like, convex and isotropy with higher bone volume. Histology confirmed the finding of lower porosity at osteochondral junction and increased bone volume. Immunohistochemistry revealed that the early OA subchondralbone change may be due to elevated level of osteoblast differentiation. Conclusions:Subchondral bone ultrastructure change occurred at early stage of OA ahead of microscopiccartilage degeneration, which may further impair articular cartilage. It was possibly related to elevated level of osteoblast differentiation.

 

 

通讯作者:Chiu, K.-Y.; Queen Mary Hospital, New Clinical Building, Pokfulam, Hong Kong; email:pkychiu@hku.hk
学科代码:整形外科学   关键词:Early stage osteoarthritis; Gu
来源: Scopus
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