在手术前后的非小细胞肺癌患者外周血中的细胞部分检出生存素与VEGF mRNA的临床意义

Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China

Purpose: The aim of this study was to evaluate the predictive and prognostic value of peripheral blood survivin and VEGF mRNA expression levels in non-small cell lung cancer (NSCLC) patients. Patients and methods: Fifty-eight patients with stage I-IIIA NSCLC who underwent surgical resection were enrolled in this study. Thirty-six patients with benign lung disease (BLD) entered this study as control group. Quantitative real-time PCR was used to detect survivin and VEGF mRNA levels in the cell fraction of peripheral blood in NSCLC patients before and after surgery and BLD patients. The relationship between blood survivin and VEGF mRNA levels and patients clinicopathologic parameters and prognostic factors were investigated. Results: The levels of survivin and VEGF mRNA were decreased significantly after surgery in NSCLC patients (P=0.024 and P=0.012 respectively). Tumor recurrence was significantly more frequent in NSCLC patients with survivin and VEGF mRNA positivity postoperation than in patients without (P=0.003 and P=0.006, respectively). Patients with survivin or VEGF mRNA positivity postoperation had markedly shorter disease-free survival (DFS) and overall survival (OS) than patients without (P=0.023 and P=0.016 for survivin; P=0.031 and P=0.025 for VEGF, respectively). Multivariate analysis showed that survivin positivity preoperation (P=0.026, P=0.041, respectively) and postoperation (P=0.003, P=0.005, respectively) and VEGF mRNA positivity postoperation (P=0.007, P=0.009, respectively) were independently associated with DFS and OS. Conclusion: Although the levels of surviving and VEGF mRNA were decreased significantly after surgery, postoperative detections of survivin and VEGF mRNA by quantitative real-time PCR could be used as tools to monitor tumor recurrence and predict prognosis. © 2013 Elsevier Ireland Ltd.

Li, J.; Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, 438 North Jiefang Road, Zhenjiang 212001, China;