Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure
2012-10-22 14:15点击:304次发表评论
作者:Liu, X.-M.a, Ding, G.-L.a, Jiang, Y.a, Pan, H.-J.a
机构: 浙江大学医学院附属妇产科医院生殖内分泌科
期刊: J CLIN ENDOCR METAB2012年10月10期97卷

Background: Low expression levels of S100A11 proteins were demonstrated in the placental villous tissue of patients with early pregnancy loss, and S100A11 is a Ca 2+-binding protein that interprets the calcium fluctuations and elicits various cellular responses. Objectives: The objective of the study was to determine S100A11 expression in human endometrium and its roles in endometrial receptivity and embryo implantation. Methods: S100A11 expression in human endometrium was analyzed using quantitative RT-PCR, Western blot, and immunohistochemical techniques. The effects of S100A11 on embryo implantation were examined using in vivo mouse model, and JAr (a human choriocarcinoma cell line) spheroid attachment assays. The effects of endometrial S100A11 on factors related to endometrial receptivity and immune responses were examined. Using a fluorescence method, we examined the changes in cytosolic Ca 2+ and Ca 2+ release from intracellular stores in epidermal growth factor (EGF)-treated endometrial cells transfected with or without S100A11 small interfering RNA. Results: S100A11 was expressed in human endometrium. S100A11 protein levels were significantly lower in endometrium of women with failed pregnancy than that in women with successful pregnancy outcomes. The knockdown of endometrial S100A11 not only reduced embryo implantation rate in mouse but also had adverse effects on the expression of factors related to endometrial receptivity and immune responses in human endometrial cells. Immunofluorescence analysis showed that S100A11 proteins were mainly localized in endoplasmic reticulum. The EGF up-regulated endometrial S100A11 expression and promoted the Ca 2+ uptake and release from Ca 2+ stores, which was inhibited by the knockdown of S100A11. Conclusions: Endometrial S100A11 is a crucial intermediator in EGF-stimulated embryo adhesion, endometrium receptivity, and immunotolerance via affecting Ca 2+ uptake and release from intracellular Ca 2+ stores. Down-regulation of S100A11 may cause reproductive failure. Copyright © 2012 by The Endocrine Society.

Department of Reproductive Endocrinology, Zhejiang Women's Hospital, Zhejiang University, Hangzhou 310006, China

通讯作者:Sheng, J.-Z.; Department of Pathology and Pathophysiology, School of Medicine, Zhejiang University, Hangzhou 310058, China; email:shengjz@zju.edu.cn
学科代码:内分泌学与糖尿病   关键词:S100A11
来源: Scopus
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