Principles of Cloning

《克隆》是克隆医学界的权威著作，第二版进行了全面的修订。本书介绍克隆的生物学机制，并讨论当今时代克隆在基础生物学、农业、生物技术和医学领域的应用以将来的前景。《克隆》以介绍克隆的历史和理论作为开端，向读者呈现精密控制的检测技术、核转移、基因修饰和克隆动物的妊娠和新生克隆动物的护理。克隆动物有多种种类，包括小鼠、绵羊、牛，科学家们甚至考虑对非哺乳类动物进行克隆。
本书的作者在克隆技术方面有大量的突破。如2012年诺贝尔生理学和医学奖获得者John Gurdon博士首次为人们展示了分化细胞的克隆；Keith Campbell和Ian Wilmut博士首次从体细胞中克隆出哺乳动物；Michael West和Robert Lanza博士发现克隆可以重启生物钟；Rudolf Jaenisch博士将末分化细胞克隆产生新细胞，Jose Cibelli博士首次从分化细胞中克隆出转基因牛。本书的大部分作者是当今每一种类克隆动物研究的主要研究者，在各自领域享有权威。
 

Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species-including mice, sheep, cattle, and non-mammals-is considered as well.
  The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine - Dr John Gurdon; the cloning of the first mammal from a somatic cell - Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual - Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell - Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas.