三萜酸的抗增生活性与人类细胞凋亡诱导、细胞周期暂停、细胞迁移抑制和细胞凋亡蛋白酶激活相关

Antiproliferative activity of rosamultic acid is associated with induction of apoptosis, cell cycle arrest, inhibition of cell migration and caspase activation in human gastric cancer (SGC-7901) cells
2015-08-18 08:23点击:154次发表评论
作者:Sui, C.-G. , Meng, F.-D., Li, Y., Jiang, Y.-H.
机构: 中国医科大学 癌症研究所分子肿瘤学系
期刊: Phytomedicine2015年7月9期22卷

Background: Gastric cancer is the second leading cause of cancer related deaths after lung cancer globally. Among natural products, natural triterpenes represent a structurally diverse group of organic compounds with potent antitumor activity. Purpose: The objective of the present research work demonstrated the antiproliferative and apoptotic activity of rosamultic acid, a natural triterpenoid, in human gastric cancer (SGC-7901) cells. Its effect on cellular morphology, cell cycle arrest, DNA fragmentation and expression levels of caspase-3, caspase-8 and caspase-9 were also determined. Methods: Antiproliferative activity of rosamultic acid was evaluated by MTT assay. Phase contrast, fluorescence microscopy as well as flow cytometry using Hoechst 33342, acridine orange/ethidium bromide and Annexin V-FITC as cellular probes were used to evaluate the induction of apoptosis by rosamultic acid. Protein level expressions were analyzed by western blot analysis. Results: The results revealed that rosamultic acid induced dose-dependent as well as time dependent cytotoxic effects in SGC-7901 gastric cancer cells. It also led to a reduction in clonogenic activity along with inhibiting the cell migration. Characteristic features of apoptosis induced by rosamultic acid were observed and quantified. Cell cycle arrest at sub-G1 phase was induced by rosamultic acid along with downregulation of expression levels of CDK4, CDK6 and cyclin D1. Rosamultic acid also significantly led to the activation of caspase-3, -8 and -9 during the 48 h treatment along with cleaving PARP in a dose-dependent manner. DNA fragmentation following rosamultic acid treatment was also observed in these cells. Conclusion: The current study strongly reveals that rosamultic acid inhibits gastric cancer proliferation by inducing apoptosis mediated through cell cycle arrest, downregulation of cell cycle related protein expressions, inhibition of cell migration, DNA damage, and activation of caspases. © 2015 Elsevier GmbH. All rights reserved.

 

通讯机构:Molecular Oncology Department of Cancer Research Institution, China Medical University, No. 155 North of Nanjing Street, Heping District, Shengyang City, Liaoning, China
学科代码:内科学 补充和替代医学 药学   关键词:三萜酸 抗增生活性 人类细胞凋亡 细胞周期 细胞迁移抑制 细胞凋亡蛋白酶激活 ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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