烟草烟雾提取物对COPD患者气道平滑肌细胞的影响

Effects of cigarette smoke extract on human airway smooth muscle cells in COPD
作者:Chen, L.ab, Ge, Q.bc, Tjin, G.bd, Alkhouri, H.e, Deng, L.af, Brandsma, C.-A.g, Adcock, I.h, Timens, W.g, Postma, D.i, Burgess, J.K.bcd, Black, J.L.bc, Oliver, B.G.G.bj
机构: 重庆大学生物工程学院 生物流变科学与技术教育部重点实验室
期刊: EUR RESPIR J2014年9月3期44卷

We hypothesised that the response to cigarette smoke in airway smooth muscle (ASM) cells from smokers with chronic obstructive pulmonary disease (COPD) would be intrinsically different from smokers without COPD, producing greater pro-inflammatory mediators and factors relating to airway remodelling. ASM cells were obtained from smokers with or without COPD, and then stimulated with cigarette smoke extract (CSE) or transforming growth factor-β1. The production of chemokines and matrix metalloproteinases (MMPs) were measured by ELISA, and the deposition of collagens by extracellular matrix ELISA. The effects of CSE on cell attachment and wound healing were measured by toluidine blue attachment and cell tracker green wound healing assays. CSE increased the release of CXCL8 and CXCL1 from human ASM cells, and cells from smokers with COPD produced more CSE-induced CXCL1. The production of MMP-1, -3 and -10, and the deposition of collagen VIII alpha 1 (COL8A1) were increased by CSE, especially in the COPD group which had higher production of MMP-1 and deposition of COL8A1. CSE decreased ASM cell attachment and wound healing in the COPD group only. ASM cells from smokers with COPD were more sensitive to CSE stimulation, which may explain, in part, why some smokers develop COPD. Copyright ©ERS 2014.

通讯作者:Key Laboratory of Biorheological Science and Technology, Bioengineering College, Chongqing University, Shapingba, Chongqing, China
学科代码:呼吸病学   关键词:烟草烟雾;COPD患者;气道平滑肌;细胞; ,中国作者重要发表 爱思唯尔医学网, Elseviermed
来源: Scopus
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